The entrance to the first chamber where a jinchūriki attempts to control one's tailed beast has a sculpture of the Two-Tails' head with a switch inside its mouth, only accessible by entering head first. The chamber is designed to hold a tailed beast, in case the jinchūriki failed to master control and allow the beast to be reborn, and lock it up until a new jinchūriki comes forth. Chakra Isolation Chamber
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Hokage: Naraku Otsutsuki 
Kazekage: Munakata Koshimaru 
Mizukage: Izuna Nobunari
Raikage: Kin Hayata 
Tsuchikage: N/A 
Kōkage: Agito Shikigami 
Sōkage: N/A 
Rōkage: Kozai Nishiki 
Yōkage: N/A 
Hikage: N/A